Lipid and Salt Effects on Carbocyanine Dye-induced Photo-voltages in Bilayer Membranes.

Johnny A. Baker; John R. Duchek; Rebecca L. Hooper; Ryan J. Koftan; Jay S. Huebner

doi:10.1016/0005-2736(79)90026-9

Lipid and Salt Effects on Carbocyanine Dye-induced Photo-voltages in Bilayer Membranes.

Johnny A. Baker, John R. Duchek, Rebecca L. Hooper, Ryan J. Koftan, Jay S. Huebner

University of North Florida

Research output: Contribution to journal › Article › peer-review

Abstract

Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3′-dimethyl-2,2′-oxacarbocyanine chloride (diO-C1-3-Cl), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.

Original language	American English
Pages (from-to)	1-10
Number of pages	10
Journal	Biochimica et Biophysica Acta
Volume	553
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(79)90026-9
State	Published - May 3 1979

Disciplines

Analytical Chemistry
Biochemistry

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title = "Lipid and Salt Effects on Carbocyanine Dye-induced Photo-voltages in Bilayer Membranes.",

abstract = "Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3′-dimethyl-2,2′-oxacarbocyanine chloride (diO-C1-3-Cl), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.",

author = "Baker, \{Johnny A.\} and Duchek, \{John R.\} and Hooper, \{Rebecca L.\} and Koftan, \{Ryan J.\} and Huebner, \{Jay S.\}",

note = "Baker, J. A., Duchek, J. R., Hooper, R. L., Koftan, R. J., \& Huebner, J. S. (1979). Lipid and salt effects on carbocyanine dye-induced photo-voltages in bilayer membranes. Biochimica et biophysica acta, 553(1), 1–10. https://doi.org/10.1016/0005-2736(79)90026-9",

year = "1979",

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doi = "10.1016/0005-2736(79)90026-9",

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T1 - Lipid and Salt Effects on Carbocyanine Dye-induced Photo-voltages in Bilayer Membranes.

AU - Baker, Johnny A.

AU - Duchek, John R.

AU - Hooper, Rebecca L.

AU - Koftan, Ryan J.

AU - Huebner, Jay S.

N1 - Baker, J. A., Duchek, J. R., Hooper, R. L., Koftan, R. J., & Huebner, J. S. (1979). Lipid and salt effects on carbocyanine dye-induced photo-voltages in bilayer membranes. Biochimica et biophysica acta, 553(1), 1–10. https://doi.org/10.1016/0005-2736(79)90026-9

PY - 1979/5/3

Y1 - 1979/5/3

N2 - Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3′-dimethyl-2,2′-oxacarbocyanine chloride (diO-C1-3-Cl), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.

AB - Voltage transients induced by flash illumination of bilayer membranes with sorbed dye, 3,3′-dimethyl-2,2′-oxacarbocyanine chloride (diO-C1-3-Cl), vary with membrane lipid composition and aqueous solution salt concentration. The voltage transients are probably induced by physical movements of sorbed dye molecules following photo-isomerization. Increased salt (NaCl and NaBr) concentrations in the aqueous solutions reduced the photo-voltage amplitudes by reducing the amount of dye sorbed to the membranes, and by decreasing the effective membrane thickness. The photo-voltage risetimes and falltimes varied systematically with salt concentration and membrane lipid composition, reflecting structural changes in the membrane's surface layer.

UR - http://www.ncbi.nlm.nih.gov/pubmed/454581

U2 - 10.1016/0005-2736(79)90026-9

DO - 10.1016/0005-2736(79)90026-9

M3 - Article

SN - 0006-3002

VL - 553

SP - 1

EP - 10

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

IS - 1

ER -

Lipid and Salt Effects on Carbocyanine Dye-induced Photo-voltages in Bilayer Membranes.

Abstract

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