MOESM5 of Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains

Additional file 5: Movie 1. The central nervous system of an H. exemplaris embryo. The movie shows a laterally mounted 45 hpl embryo. Anterior is toward the top. The ventral side is facing toward the right. Nuclei are stained with DAPI (blue). The nervous system is stained with a fluorescent secondary antibody bound to a β-tubulin antibody (red). The movie was produced from 35 slices of a Z-series collected on a confocal laser scanning microscope. Slice 8 shows a neurite cluster that extends from the outer brain region (ob) to the first trunk ganglion. In the region between the first trunk ganglion and the head, this neurite cluster is referred to as the outer connective [47, 64]. In slices 10–17, the neurite cluster widens and more clearly represents brain neuropil. In slice 18, the region where the outer brain neuropil and the inner brain neuropil (ib) meet to give rise to the dorsal brain neuropil (dnp) is visible [64]. The inner brain neuropil extends from the dorsal position to a ventral position within the head (slices 18–28). At a ventral position within the head, the inner brain neuropil gives rise to the inner connective, which connects to the first trunk ganglion [47, 64]. The preoral brain commissure (prc), which connects the left and right inner brain regions above the mouth, is visible in slices 24–35 [64]. The post oral brain commissure, which connects the left and right inner brain regions below the mouth, is visible in slices 30–35 [64]. The right legs (L1–L4), trunk ganglia (ga1–ga4), and right ganglion connective (cn) are also visible.